5 Simple Statements About attune flow cytometer Explained
5 Simple Statements About attune flow cytometer Explained
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Knowledge-enriched characterization of mobile populations. Morphological details from photographs can add to your richness of flow cytometry facts. For instance, the figure shows an in any other case regular apoptosis assay working with Annexin V and PI, incorporating mobile imaging to characterize cells in Every populace to expose morphologically unique characteristics. These insights could not are already obtained from multiplex staining by itself.
As samples are acquired about the Attune CytPix Flow Cytometer, the large-speed brightfield camera captures and outlets images of detected situations in a charge around six,000 visuals for each next, dependant upon the flow rate and image sizing. For higher adaptability, Attune Cytometric Application allows you to modify the impression capture frequency as necessary and choose a selected gate for picture seize, if essential.
Combine acoustic focusing technologies with higher-parameter Investigation for flexible spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing with a significant-speed brightfield digicam for simultaneous superior throughput flow cytometry and higher resolution brightfield imaging.
"An image isn't pretty really worth a thousand dots, nonetheless it seriously helps to see photographs. It is great to determine photos that correlate with staining. By way of example, AnnexinV-optimistic cells which can be morphologically diverse than wholesome cells, or CD14+ monocytes which can be larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a qualified person has gated singlets confidently. Right after analyzing the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate contains greater than four% aggregates.
Optimizing gating strategy. Even sturdy guide singlet gating is mistake-prone and continues to be a subjective choice level in Pretty much all flow cytometry assays. Imaging may be used to verify and modify gates to include only single cells of curiosity.
Our experts will design and style panels in your case as over and over you would like—free of charge, no product or service purchase desired.
The Attune NxT cytometer may be the reliable workhorse instrument your lab can depend on for consistent and trustworthy general performance. The Attune CytPix design contains a high-pace brightfield camera and AI-driven graphic Investigation program, with modern upgrades like device Discovering equipment and an intuitive consumer interface, enabling Improved details precision, precision, and more rapidly insights into sample biology.
Potentially most of all, these activities incorporate cells of Obviously unique phenotypes which could lead to incorrect conclusions regarding double favourable gatherings (particularly in uncommon populations).
AI-pushed automated picture Assessment While using the Attune Cytometric Program interprets celebration characteristics into distinctive morphology parameters that could be combined with standard fluorescence and scatter parameters to expose extremely reproducible populace adjustments and unique subsets.
We previously demonstrated the power of imaging Auto-T/Ramos mobile interactions. Allow’s evaluate just the inhabitants of finest desire, the double good activities, To find out more. We can now use prolonged morphological parameters (in this article, circularity and skewness of depth) to further more examine the options of these populations and refine gating on these read more functions, increasing facts robustness.
Constructed-in dimensionality reduction resources enhanced resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify anticipated population resolution in lysed whole human blood. Consumer can see activities from Every single cluster for visual affirmation of cell phenotypes.
No clean/no lyse leukocyte Evaluation with imaging. Cells had been acquired from 24-hour-outdated blood by dilution in one mM EDTA ( Professional UX/UI permits customers to train AI products for expanded apps. (A) The “Teach Design” feature presents annotation applications and actual-time design education precision.
Gating on these prolonged parameters helps you to immediately and properly detect populations of interest and confirm gating approach with little if any guide evaluate. The Attune computer software delivers usage of point out-of-the-artwork machine Finding out Investigation applications inside of an expertly built consumer interface, making it extremely convenient to use.
The Attune Xenith cytometer can be a higher-parameter spectral analyzer, delivering both spectral unmixing and conventional flow abilities for extensive and functional cell Examination.
The Attune CytPix Flow Cytometer permits users to visualize samples all through acquisition, confirm the precision of gating procedures, and support benefits with responsible documentation for publication applications.
Right here, we show that by gating strategies utilizing image-based quantitative parameters can distinguish interacting cells from coincident functions to extra precisely evaluate interacting cells.